Publications

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2021

Kim J and Kwon YC* (2021), Manuscript in Preparation.

 

Copeland CE, Langlois A, Kim J, and Kwon YC* (2021) The cell-free system: A new apparatus for affordable, sensitive, and portable healthcare. Submitted. 

2020

Kim J, Copeland CE, Seki K, Vögeli B, and Kwon YC* (2020) Tuning the cell-free protein synthesis system for biomanufacturing of monomeric human filaggrin. Frontiers in Bioengineering and Biotechnology 8:5990341. Link

Vaitaitis VJ, Dunham ME, Kwon YC, Mayer WC, Evans AK, Baker AJ, Walker KD, Cespedes GD, Stanley A, and Opiri M (2020) A surgical simulator for tympanostomy tube insertion incorporating capacitive sensing technology to track instrument placement. Otolaryngology-Head and Neck Surgery 162(3), 343-345. Link

2019

Kim J1, Copeland CE1, Padumane SR, and Kwon YC* (2019) A crude extract preparation and optimization from a genomically engineered Escherichia coli for the cell-free protein synthesis system: Practical laboratory guideline. Methods and Protocols 2(3), 68. Link (1: equal contribution)

2018

Jin X, Kightlinger W, Kwon YC, and Hong SH (2018) Rapid production and characterization of antimicrobial colicins using Escherichia coli-based cell-free protein synthesis. Synthetic Biology 3(1), ysy004. Link

Martin RW, Des Soye BJ, Kwon YC, Kay JE, Davis RG, Thomas PM, Majewska NI, Chen CX, Marcum RD, Weiss MG, Stoddart AE, Amiram M, Ranji AK, Patel JR, Isaacs FJ, Kelleher NL, Hong SH, and Jewett MC (2018) Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids. Nature Communications 9(1), 1203. Link

Casini A, Chang FY, Eluere R, King AM, Young EM, Dudley QM, Karim A, Pratt K, Bristol C, Forget A, Ghodasara A, Warden-Rothman R, Gan R, Cristofaro A, Borujeni AE, Ryu MH, Li J, Kwon YC, Wang H, Tatsis E, Rodriques-Lopez C, O’Connor S, Medema MH, Fischbach MA, Jewett MC, Voigt CA, and Gordon DB (2018) A pressure test to make 10 molecules in 90 days: External evaluation of methods to engineer biology. Journal of the American Chemical Society 140(12), 4302-4316. Link 

2006-2017

Li J, Wang H, Kwon YC, and Jewett MC (2017) Establishing a high yielding Streptomyces-based cell-free protein synthesis system. Biotechnology and Bioengineering 114(6), 1343-1353. Link

Hong SH1, Kwon, YC1, Martin RW, Des Soye BJ, de Paz AM, Swonger KN, Ntai I, Kelleher NL, and Jewett MC (2015) Improving cell-free protein synthesis through genome engineering of Escherichia coli lacking release factor 1. ChemBioChem 16(5), 844-853. Link (1: equal contribution)

Kwon YC and Jewett MC (2015) High-throughput preparation methods of crude extract for robust cell-free protein synthesis. Scientific Reports 5, 8663. Link 

Hong SH, Kwon YC, and Jewett MC (2014) Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis. Frontiers in Chemistry 2, 34. Link

Kwon YC, Oh IS, Lee N, Lee KH, Yoon YJ, Lee EY, Kim BG, and Kim DM (2013) Integrating cell-free biosyntheses of heme prosthetic group and apoenzyme for the synthesis of functional P450 monooxygenase. Biotechnology and Bioengineering 110(4), 1193-1200. Link

Lee SH1, Kwon YC1, Kim DM, and Park CB (2013) Cytochrome P450-catalyzed O-dealkylation coupled with photochemical NADPH regeneration. Biotechnology and Bioengineering 110(2), 383-390. Link (1: equal contribution)

Kim HC, Kwon YC, Lee KH, and Kim DM (2011) Multi-hour translation of mRNA in a cell-free system. Biotechnology and Bioprocess Engineering 16, 1152-1156. Link

Kwon YC, Lee KH, Kim HC, Han K, Seo JH, Kim BG, and Kim DM (2010) Cloning-independent expression and analysis of omega-transaminase by use of cell-free protein synthesis system. Applied and Environmental Microbiology 76(18), 6295-6298. Link

Lee KH, Kwon YC, Yoo SJ, and Kim DM (2010) Ribosomal synthesis and in situ isolation of peptide molecules in a cell-free translation system. Protein Expression and Purification 71(1), 16-20. Link

Kwon YC1, Hahn GH1, Huh KM, and Kim DM (2008) Synthesis of functional proteins using Escherichia coli extract entrapped in calcium alginate microbeads. Analytical Biochemistry 373(2), 192-196. Link (1: equal contribution)

Kim TW, Oh IS, Keum JW, Kwon YC, Byun JY, Lee KH, Choi CY, and Kim DM (2007) Prolonged cell-free protein synthesis using dual energy sources: Combined use of creatine phosphate and glucose for the efficient supply of ATP and accumulation of phosphate. Biotechnology and Bioengineering 97(6), 1510-1515. Link

Keum JW, Ahn JH, Choi CY, Lee KH, Kwon YC, and Kim DM (2006) The presence of a common downstream box enables the simultaneous expression of multiple proteins in an extract. Biochemical and Biophysical Research Communications 350(3), 562-567. Link

Kwon YC, Park KM, and Kim DM (2006) Rapid preparation of truncated transaminases using a PCR-based cell-free protein synthesis system. The Korean Society for Biotechnology and Bioengineering Journal 21, 315-318. 

Book Chapters

Kwon YC, Song JK, and Kim DM (2014) Cloning-independent expression and screening of enzymes using cell-free protein synthesis system. Methods in Molecular Biology 1118, 97-108, in “Cell-Free Protein Synthesis,” Kirill Alexandrov and Wayne A. Johnston, editor. New York City, NY, Humana Press. Link